In Vivo Imaging of Endogenously Produced HClO in Zebrafish and Mice Using a Bright, Photostable Ratiometric Fluorescent Probe
- Authors
- Duan, Chong; Won, Miae; Verwilst, Peter; Xu, Junchao; Kim, Hyeong Seok; Zeng, Lintao; Kim, Jong Seung
- Issue Date
- 19-3월-2019
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.91, no.6, pp.4172 - 4178
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 91
- Number
- 6
- Start Page
- 4172
- End Page
- 4178
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/66637
- DOI
- 10.1021/acs.analchem.9b00224
- ISSN
- 0003-2700
- Abstract
- A high brightness red fluorescent probe (S-BODIPY) has been developed for the sensitive and specific imaging of HClO/ClO- in vitro and in vivo. This probe exhibits some distinctive features such as excellent resistance to photobleaching, a high fluorescence brightness, high selectivity, as well as a good biocompatibility. Upon oxidation of the thioether group into sulfoxide, the probe showed a noticeable ratiometric fluorescence response toward ClO- with fast response (within 30 s) and a low detection limit (59 nM). The probe demonstrated the successful imaging of exogenous and endogenous HClO/ClO- in living HeLa cells, zebrafish, and mice with high signal-to-noise ratios. S-BODIPY allows for the real-time monitoring the level of ClO- in living cells by ratiometric fluorescence imaging, opening up exciting prospects to brightness and good photostability for in vivo imaging. develop red and even near-infrared BODIPYs with high
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