Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding

Authors
Kim, Sang HoonNam, OnyouJin, EonSeonGu, Man Bock
Issue Date
1-1월-2019
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
A cognate pair of aptamers; Sandwich-type binding; Electrochemical biosensor; Coccolith; Chronoamperometry; Type 2 diabetes
Citation
BIOSENSORS & BIOELECTRONICS, v.123, pp.160 - 166
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
123
Start Page
160
End Page
166
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/68368
DOI
10.1016/j.bios.2018.08.021
ISSN
0956-5663
Abstract
In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers. The morphological, electrochemical characteristics and the performances of both the CME-SPGE and the completely fabricated sandwich type aptasensor were investigated by SEM, EDAX, cyclic voltammetry, and chronoamperometry. Due to the synergic effect of a cognate pair of aptamers on CME-SPGE, this newly developed sandwich-type electrochemical biosensor for Vaspin showed high specificity, and good sensitivity with a limit of detection (LOD) of 298 pM, along with more widen the linear range. To the best of our knowledge, this is the first report about the use of a coccolith modified electrode with a cognate pair aptamer resulting in sandwich-type binding in an electrochemical biosensor. With the advantages of using highly-structured biomineral microparticles and a cognate pair of aptamers, this new study may pave the innovative way to design a novel sandwich-type electrochemical aptasensor platform.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Gu, Man Bock photo

Gu, Man Bock
융합생명공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE