Inhibition of lactate dehydrogenase A suppresses inflammatory response in RAW 264.7 macrophages
DC Field | Value | Language |
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dc.contributor.author | Song, Yoo-Jeong | - |
dc.contributor.author | Kim, Ahyeon | - |
dc.contributor.author | Kim, Goon-Tae | - |
dc.contributor.author | Yu, Han Young | - |
dc.contributor.author | Lee, Eun-So | - |
dc.contributor.author | Park, Mi Jin | - |
dc.contributor.author | Kim, Young-Jun | - |
dc.contributor.author | Shim, Soon-Mi | - |
dc.contributor.author | Park, Tae-Sik | - |
dc.date.accessioned | 2021-09-01T22:26:35Z | - |
dc.date.available | 2021-09-01T22:26:35Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/68793 | - |
dc.description.abstract | Lactate is an important metabolite in cellular metabolism and fluctuates in certain disease conditions including cancer and immune diseases. It was hypothesized that a decrease in lactate would modulate the inflammatory response elicited by lipopolysaccharides (LPS) in macrophages. When RAW 264.7 macrophages were treated with FX11, a specific lactate dehydrogenase (LDHA) inhibitor, the expression of the cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) was downregulated due to reduced cellular lactate levels. Genetic suppression of LDHA by small interfering RNA (siRNA) downregulated the LPS-activated expression of interleukin (IL)-6, iNOS, and COX-2, and reduced the production of IL-6 and nitrites. Pharmacological and genetic suppression of LDHA inhibited the phosphorylation of p38 mitogen-activated protein kinase. Microarray gene expression profile demonstrated that the genes involved in cell proliferation and inflammation were mainly altered by siRNA-mediated LDHA suppression. Collectively, the present observations suggest that lactate may be an important metabolite and implicated in regulation of inflammatory response. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | EXPRESSION | - |
dc.subject | GLYCOLYSIS | - |
dc.subject | METABOLISM | - |
dc.subject | ACTIVATION | - |
dc.subject | CANCER | - |
dc.title | Inhibition of lactate dehydrogenase A suppresses inflammatory response in RAW 264.7 macrophages | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Jun | - |
dc.identifier.doi | 10.3892/mmr.2018.9678 | - |
dc.identifier.scopusid | 2-s2.0-85059529570 | - |
dc.identifier.wosid | 000454431000069 | - |
dc.identifier.bibliographicCitation | MOLECULAR MEDICINE REPORTS, v.19, no.1, pp.629 - 637 | - |
dc.relation.isPartOf | MOLECULAR MEDICINE REPORTS | - |
dc.citation.title | MOLECULAR MEDICINE REPORTS | - |
dc.citation.volume | 19 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 629 | - |
dc.citation.endPage | 637 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GLYCOLYSIS | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordAuthor | lactate | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | lactate dehydrogenase | - |
dc.subject.keywordAuthor | mitogen-activated protein kinase | - |
dc.subject.keywordAuthor | cytokines | - |
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