Characterization of inhibitory constituents of NO production from Catalpa ovata using LC-MS coupled with a cell-based assay
- Authors
- Park, Sangmin; Shin, Hyeji; Park, Yeeun; Choi, Ilgyu; Park, Byoungduck; Lee, Ki Yong
- Issue Date
- 10월-2018
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Catalpa ovata; Bignoniaceae; Chemical profile; LC-QTOF MS/MS; Anti-inflammatory; NO production; LPS
- Citation
- BIOORGANIC CHEMISTRY, v.80, pp.57 - 63
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOORGANIC CHEMISTRY
- Volume
- 80
- Start Page
- 57
- End Page
- 63
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/72643
- DOI
- 10.1016/j.bioorg.2018.05.023
- ISSN
- 0045-2068
- Abstract
- An effective screening method for inhibitors of NO production in natural products using LC-QTOF MS/MS coupled with a cell-based assay was proposed. The ethyl acetate fraction of Catalpa ovata exhibited a strong inhibitory effect on NO production in lipopolysaccharide-induced BV2 microglia cells. We attempted to identify the active constituents of C. ovata by using LC-QTOF MS/MS coupled with a cell-based assay. Peaks at approximately 14-15 min on the MS chromatogram were estimated to be the bioactive constituents. A new iridoid compound, 6-O-trans-feruloyl-3 beta-hydroxy-7-deoxyrehamaglutin A (4), and nine known compounds (1-3, 5-10) were isolated from the ethyl acetate fraction of C. ovata by repeated column chromatography. Compounds 3, 4, 5, 7, and 8 significantly attenuated lipopolysaccharide-stimulated NO production in BV2 cells. Our results indicate that LC-QTOF MS/MS coupled with a cell-based NO production inhibitory assay successfully predicted active compounds without a time-consuming isolation process.
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Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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