Attenuation of inflammation and cartilage degradation by sulfasalazine-containing hyaluronic acid on osteoarthritis rat model
- Authors
- Kim, Sung Eun; Lee, Jae Yong; Shim, Kyu-Sik; Lee, Sunghee; Min, Kyoengwoo; Bae, Ji-Hoon; Kim, Hak-Jun; Park, Kyeongsoon; Song, Hae-Ryong
- Issue Date
- 15-7월-2018
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Sulfasalazine; Hyaluronic acid; Osteoarthritis
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.114, pp.341 - 348
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 114
- Start Page
- 341
- End Page
- 348
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/74340
- DOI
- 10.1016/j.ijbiomac.2018.03.059
- ISSN
- 0141-8130
- Abstract
- The aim of this study was to investigate the effects of a sulfasalazine-containing hyaluronic acid (SASP/HA) systems on in vitro anti-inflammation and the alleviation of cartilage degradation in both lipopolysaccharide (LPS)-stimulated synoviocytes and a rat model of monosodium iodoacetate (MIA)-induced osteoarthritis (OA). The SASP/HA resulted in long-term release of SASP from the SASP/HA for up to 60 days in a sustained manner. In vitro studies performed using real-time polymerase chain reaction (PCR) assay revealed that the SASP/HA was able to effectively and dose-dependently inhibit the mRNA expression levels of pro-inflammatory cytokines such as matrix metalloproteinases-3 (MMP-3), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in LPS-stimulated synoviocytes. In vivo studies showed that intra articular injection of SASP/HA greatly reduced the MR-stimulated mRNA expression of MMP-3, COX-2, IL-6, and TNF-alpha in blood. Furthermore, these significant anti-inflammatory effects of SASP/HA contributed markedly to the alleviation of progression of MIA-induced OA and cartilage degradation, as demonstrated by X-ray, micro-computed tomography (micro-CT), gross findings, and histological evaluations. Therefore, our findings indicated that the long-term and sustained delivery of SASP using HA can play a therapeutic role in alleviating inflammation as well as protecting against cartilage damage in OA. (C) 2018 Elsevier B.V. All rights reserved.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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