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Biophysical characterization of the basic cluster in the transcription repression domain of human MeCP2 with AT-rich DNA
- Authors
- Ul Mushtaq, Ameeq; Lee, Yejin; Hwang, Eunha; Bang, Jeong Kyu; Hong, Eunmi; Byun, Youngjoo; Song, Ji-Joon; Jeon, Young Ho
- Issue Date
- 1-Jan-2018
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Methyl-CpG-binding protein 2; Transcription repression domain; Basic cluster; AT-Hook; AT-rich DNA binding
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.495, no.1, pp.145 - 150
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 495
- Number
- 1
- Start Page
- 145
- End Page
- 150
- ISSN
- 0006-291X
- Abstract
- MeCP2 is a chromatin associated protein which is highly expressed in brain and relevant with Rett syndrome (RTT). There are AT-hook motifs in MeCP2 which can bind with AT-rich DNA, suggesting a role in chromatin binding. Here, we report the identification and characterization of another AT-rich DNA binding motif (residues 295 to 313) from the C-terminal transcription repression domain of MeCP2 by nuclear magnetic resonance (NMR) and isothermal calorimetry (ITC). This motif shows a micromolar affinity to AT-rich DNA, and it binds to the minor groove of DNA like AT-hook motifs. Together with the previous studies, our results provide an insight into a critical role of this motif in chromatin structure and function. (C) 2017 Elsevier Inc. All rights reserved.
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Related Researcher
- JEON, Young Ho
- College of Pharmacy (Department of Pharmaceutical Science)