Optimization of hexanoic acid production in recombinant Escherichia coli by precise flux rebalancing
- Authors
- Kim, Seong Gyeong; Jang, Sungho; Lim, Jae Hyung; Jeon, Byoung Seung; Kim, Jungyeon; Kim, Kyoung Heon; Sang, Byoung-In; Jung, Gyoo Yeol
- Issue Date
- Jan-2018
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Hexanoic acid; Flux rebalancing; Acetyl-CoA acetyltransferase; 5 ' -UTR; Acetyl-CoA transferase
- Citation
- BIORESOURCE TECHNOLOGY, v.247, pp.1253 - 1257
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 247
- Start Page
- 1253
- End Page
- 1257
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/78074
- DOI
- 10.1016/j.biortech.2017.10.014
- ISSN
- 0960-8524
- Abstract
- The aim of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-CoA branch node can substantially improve the titer and productivity of hexanoic acid in recombinant Escherichia coli strains. First, a hexanoic acid-producing E. coli strain was constructed by expressing genes encoding beta-ketothiolase (BktB) from Cupriavidus necator and acetyl-CoA transferase (ACT) from Megasphaera sp. MH in a butyric acid producer strain. Next, metabolic flux was optimized at the acetyl-CoA branch node by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four synthetic 5'- untranslated regions were designed for atoB using UTR Designer to modulate the expression level of the gene. Notably, the productivity of the optimized strain (14.7 mg/L/h) was the highest among recombinant E. coli strains in literature when using a similar inoculum size for fermentation. These results show that fine-tuning the expression level of atoB is critical for production of hexanoic acid.
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