건강기능식품 원료로서 길초근 주정 추출물의 지표성분 분석법 검증HPLC Validation of Valerian Ethanol Extract as a Functional Food
- Other Titles
- HPLC Validation of Valerian Ethanol Extract as a Functional Food
- Authors
- 조경애; 서형주; 한성희; 배송환; 신중철
- Issue Date
- 2018
- Publisher
- 한국식품영양학회
- Keywords
- Valerinan officinalis; valerenic acid; HPLC; validation; functional health food
- Citation
- 한국식품영양학회지, v.31, no.1, pp.104 - 108
- Indexed
- KCI
- Journal Title
- 한국식품영양학회지
- Volume
- 31
- Number
- 1
- Start Page
- 104
- End Page
- 108
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/78628
- DOI
- 10.9799/ksfan.2018.31.1.104
- ISSN
- 1225-4339
- Abstract
- The purpose of this study was to establish valerenic acid as a marker compound for the standardization of ethanol extract of Valerinan officinalis (valerian) root as a functional health food. We established valerenic acid as a marker compound using HPLC. HPLC was used to quantify the marker compound in the valerian extract after validation of methods with linearity, accuracy, and precision. The specificity for retention time was met by comparative analysis of the valerian extract and standard compound using HPLC. The method showed high linearity of the calibration curve with a coefficient of correlation (R2) of 0.9999. The limit of quantification (LOQ) was 10 μg/mL. The accuracy of measurement was 99.88~ 00.68% and the relative standard deviation (RSD) value was 0.59%. In addition, our analytical method yielded a 29% mean content of valerenic acid in the valerian ethanol extract. These results indicate that the established HPLC method facilitated the determination of marker compounds in the valerian extract for the standardization of health functional foods.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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