Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays
- Authors
- Lee, Jong Min; Moon, Joo Yoon; Shaker, Mohammed R.; Sun, Woong; Chung, Bong Geun
- Issue Date
- 12월-2017
- Publisher
- WILEY
- Keywords
- Hydrogel microwell array; Motoneuron differentiation; Uniform-sized neurosphere
- Citation
- ELECTROPHORESIS, v.38, no.24, pp.3161 - 3167
- Indexed
- SCIE
SCOPUS
- Journal Title
- ELECTROPHORESIS
- Volume
- 38
- Number
- 24
- Start Page
- 3161
- End Page
- 3167
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81260
- DOI
- 10.1002/elps.201700118
- ISSN
- 0173-0835
- Abstract
- We developed the photocrosslinkable hydrogel microwell arrays for uniform-sized neurosphere-mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform-sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform-sized neurospheres harvested from microwell arrays were replated into laminin-coated substrate. In parallel, uniform-sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three-dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150m in diameter) on motoneuron differentiation, showing that the largest uniform-sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform-sized neurosphere-mediated motoneuron differentiation.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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