7,10-Epoxyoctadeca-7,9-dienoic Acid: A Small Molecule Adjuvant That Potentiates beta-Lactam Antibiotics Against Multidrug-Resistant Staphylococcus aureus
- Authors
- Dasagrandhi, Chakradhar; Kim, Young-Soon; Kim, In-Hwan; Hou, Ching T.; Kim, Hak-Ryul
- Issue Date
- 12월-2017
- Publisher
- SPRINGER
- Keywords
- Furan fatty acids; 7,10-Epoxyoctadeca-7,9-dienoic acid; Synergistic antibacterial agent; beta-Lactam antibiotics; Multidrug-resistant Staphylococcus aureus
- Citation
- INDIAN JOURNAL OF MICROBIOLOGY, v.57, no.4, pp.461 - 469
- Indexed
- SCIE
SCOPUS
- Journal Title
- INDIAN JOURNAL OF MICROBIOLOGY
- Volume
- 57
- Number
- 4
- Start Page
- 461
- End Page
- 469
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81393
- DOI
- 10.1007/s12088-017-0680-2
- ISSN
- 0046-8991
- Abstract
- The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infections with multi-drug resistance needs effective and alternative control strategies. In this study we investigated the adjuvant effect of a novel furan fatty acid, 7,10-epoxyoctadeca-7,9-dienoic acid (7,10-EODA) against multidrug-resistant S. aureus (MDRSA) strain 01ST001 by disc diffusion, checker board and time kill assays. Further the membrane targeting action of 7,10-EODA was investigated by spectroscopic and confocal microscopic studies. 7,10-EODA exerted synergistic activity along with beta-lactam antibiotics against all clinical MRSA strains, with a mean fractional inhibitory concentration index below 0.5. In time-kill kinetic study, combination of 7,10-EODA with oxacillin, ampicillin, and penicillin resulted in 3.8-4.2 log(10) reduction in the viable counts of MDRSA 01ST001. Further, 7,10-EODA dose dependently altered the membrane integrity (p < 0.001) and increased the binding of fluorescent analog of penicillin, Bocillin-FL to the MDRSA cells. The membrane action of 7,10-EODA further facilitated the uptake of several other antibiotics in MDRSA. The results of the present study suggested that 7,10-EODA could be a novel antibiotic adjuvant, especially useful in repurposing beta-lactam antibiotics against multidrug-resistant MRSA.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.