Restoration of miR-29b exerts anti-cancer effects on glioblastoma
- Authors
- Shin, Jaekyung; Shim, Hyun Geun; Hwang, Taeyoung; Kim, Hyungsin; Kang, Shin-Hyuk; Dho, Yun-Sik; Park, Sung-Hye; Kim, Sang Jeong; Park, Chul-Kee
- Issue Date
- 17-11월-2017
- Publisher
- BMC
- Keywords
- Glioblastoma; miR-29b; Anti-cancer effect; Nanoparticle
- Citation
- CANCER CELL INTERNATIONAL, v.17
- Indexed
- SCIE
SCOPUS
- Journal Title
- CANCER CELL INTERNATIONAL
- Volume
- 17
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81528
- DOI
- 10.1186/s12935-017-0476-9
- ISSN
- 1475-2867
- Abstract
- Background: Glioblastoma multiforme (GBM) is known as one of the most fatal forms of cancer. MicroRNAs have been widely implicated in the regulation of mammalian development and pathogenesis. The brain-enriched miR-29 subfamilies are known to be exclusively expressed in the developing brain, and they are aberrantly down-regulated in GBM. This study aims to elucidate the role of miR-29b in GBM development and the feasibility of therapeutic targeting using conjugated nanoparticles. Methods: After confirmation of miR-29b expression levels in GBM tissues by analysis of open source data, the anticancer effect of miR-29b was tested by the introduction of syn-hsa-miR-29b-3p in the A172 GBM cell line. In vitro studies of cell viability and apoptosis and ex vivo study using GBM tissue slice cultures from 3 patients and nanoparticle delivery of miR-29b were performed. Results: We discovered an increase in apoptotic cell populations with the introduction of miR-29b in the GBM cell line. An established human-derived GBM tissue slice culture system confirmed the anticancer effect of miR29b-conjugated nanoparticles. Using PCR array, we found that exogenous miR-29b inhibits the expression of COL1A2, COL3A1, COL4A1, ELN, ITGA11, MMP24, and SPARC, which mediates an anticancer effect. Conclusions: miR-29b may serve as a putative therapeutic molecule when its expression is restored using a nanoparticle delivery system in GBM.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.