Intracellular ROS levels determine the apoptotic potential of keratinocyte by Quantum Dot via blockade of AKT Phosphorylation
- Authors
- Lee, Eun Young; Bae, Hyun Cheol; Lee, Hana; Jang, Yeonsue; Park, Yoon-Hee; Kim, Jin Hee; Ryu, Woo-In; Choi, Byeong Hyeok; Kim, Ji Hyun; Jeong, Sang Hoon; Son, Sang Wook
- Issue Date
- 11월-2017
- Publisher
- WILEY
- Keywords
- AKT; apoptosis; HSEM; keratinocyte; quantum dots; ROS
- Citation
- EXPERIMENTAL DERMATOLOGY, v.26, no.11, pp.1046 - 1052
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL DERMATOLOGY
- Volume
- 26
- Number
- 11
- Start Page
- 1046
- End Page
- 1052
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81817
- DOI
- 10.1111/exd.13365
- ISSN
- 0906-6705
- Abstract
- Quantum dots (QDs) have shown great potential for biomedical use in a broad range including diagnostic agents. However, the regulatory mechanism of dermal toxicity is poorly understood. In this study, we investigated how QDs-induced apoptosis is regulated in human keratinocytes. We also examined the effect of carboxylic acid-coated QDs (QD 565 and QD 655) on reactive oxygen species (ROS) production and apoptosis-related cellular signalling. The viability of keratinocyte was inhibited by two types of QDs in a concentration-dependent manner. QDs induce ROS production and blockade of AKT phosphorylation. Moreover, the cleavage of AKT-dependent pro-apoptotic proteins such as poly (ADP-ribose) polymerase, caspases-3 and caspases-9 was significantly increased. We also found that a decrease in cellular ROS level by ROS scavenger, N-acetylcysteine (NAC), resulting in the abolishment of QDs-induced AKT de-phosphorylation and cellular apoptosis. Interestingly, QD 655 had a more cytotoxic effect including oxidative stress and AKT-dependent apoptosis than QD 565. In addition, QD 655 had the cytotoxic potential in the human skin equivalent model (HSEM). These data show that QD-induced intracellular ROS levels may be an important parameter in QD-induced apoptosis. These findings from this study indicate that intracellular ROS levels might determine the apoptotic potential of keratinocyte by QD via blockade of AKT phosphorylation.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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