Protective effects of chebulic acid on alveolar epithelial damage induced by urban particulate matter
- Authors
- Lee, Kyung-Won; Nam, Mi-Hyun; Lee, Hee-Ra; Hong, Chung-Oui; Lee, Kwang-Won
- Issue Date
- 19-7월-2017
- Publisher
- BMC
- Keywords
- Urban particulate matter; Chebulic acid; Pulmonary alveolus; Inflammation; Alveolar barrier dysfunction
- Citation
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.17
- Indexed
- SCIE
SCOPUS
- Journal Title
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Volume
- 17
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/82812
- DOI
- 10.1186/s12906-017-1870-5
- ISSN
- 1472-6882
- Abstract
- Background: Chebulic acid (CA) isolated from T. chebula, which has been reported for treating asthma, as a potent anti-oxidant resources. Exposure to ambient urban particulate matter (UPM) considered as a risk for cardiopulmonary vascular dysfunction. To investigate the protective effect of CA against UPM-mediated collapse of the pulmonary alveolar epithelial (PAE) cell (NCI-H441), barrier integrity parameters, and their elements were evaluated in PAE. Methods: CA was acquired from the laboratory previous reports. UPM was obtained from the National Institutes of Standards and Technology, and these were collected in St. Louis, MO, over a 24-month period and used as a standard reference. To confirm the protection of PAE barrier integrity, paracellular permeability and the junctional molecules were estimated with determination of transepithelial electrical resistance, Western Blotting, RT-PCR, and fluorescent staining. Results: UPM aggravated the generation of reactive oxygen species (ROS) in PAE and also decreased mRNA and protein levels of junction molecules and barrier integrity in NCI-H441. However, CA repressed the ROS in PAE, also improved barrier integrity by protecting the junctional parameters in NCI-H411. Conclusions: These data showed that CA resulted in decreased UPM-induced ROS formation, and the protected the integrity of the tight junctions against UPM exposure to PAE barrier.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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