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Acetoin Production Using Metabolically Engineered Klebsiella pneumoniae
- Authors
- Jang, Ji-Woong; Jung, Hwi-Min; Kim, Duck Gyun; Oh, Min-Kyu
- Issue Date
- 4월-2017
- Publisher
- KOREAN INSTITUTE CHEMICAL ENGINEERS
- Keywords
- Metabolic engineering; Acetoin; 2,3-Butanediol dehydrogenase; NADH oxidase
- Citation
- KOREAN CHEMICAL ENGINEERING RESEARCH, v.55, no.2, pp.237 - 241
- Indexed
- SCOPUS
KCI
- Journal Title
- KOREAN CHEMICAL ENGINEERING RESEARCH
- Volume
- 55
- Number
- 2
- Start Page
- 237
- End Page
- 241
- ISSN
- 0304-128X
- Abstract
- Acetoin is variously applicable platform chemical in chemical and food industry. In this study, Klebsiella pneumoniae was engineered for acetoin production using metabolic engineering. From the recombinant Klebsiella pneumoniae (KMK-05) producing 2,3-butanediol, budC and dhaD genes encoding two 2,3-butanediol dehydrogenases were deleted to reduce 2,3-butanediol production. Furthermore, a transcriptional regulator, AcoK, was deleted to reduce the expression levels of acetoin degrading enzyme. Lastly, NADH oxidase was overexpressed for adjusting intracellular redox balance. The resulting strain (KJW-03-nox) produced considerable amount of acetoin, with concentration reaching 51 g/L with 2.6 g/L/h maximum productivity in 36 h fed-batch fermentation.
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