STABILIZED1 Modulates Pre-mRNA Splicing for Thermotolerance
- Authors
- Kim, Geun-Don; Cho, Young-Hee; Lee, Byeong-Ha; Yoo, Sang-Dong
- Issue Date
- 4월-2017
- Publisher
- AMER SOC PLANT BIOLOGISTS
- Citation
- PLANT PHYSIOLOGY, v.173, no.4, pp.2370 - 2382
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLANT PHYSIOLOGY
- Volume
- 173
- Number
- 4
- Start Page
- 2370
- End Page
- 2382
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/84075
- DOI
- 10.1104/pp.16.01928
- ISSN
- 0032-0889
- Abstract
- High-temperature stress often leads to differential RNA splicing, thus accumulating different types and/or amounts of mature mRNAs in eukaryotic cells. However, regulatory mechanisms underlying plant precursor mRNA (pre-mRNA) splicing in the environmental stress conditions remain elusive. Herein, we describe that a U5-snRNP-interacting protein homolog STABILIZED1 (STA1) has pre-mRNA splicing activity for heat-inducible transcripts including HEAT STRESS TRANSCRIPTION FACTORs and various HEAT SHOCK PROTEINs for the establishment of heat stress tolerance in Arabidopsis (Arabidopsis thaliana). Our cell-based splicing reporter assay demonstrated STA1 acts on pre-mRNA splicing for specific subsets of stress-related genes. Cellular reconstitution of heat-inducible transcription cascades supported the view that STA1-dependent premRNA splicing plays a role in DREB2A-dependent HSFA3 expression for heat-responsive gene expression. Further genetic analysis with a loss-of-function mutant sta1-1, STA1-expressing transgenic plants in Col background, and STA1-expressing transgenic plants in the sta1-1 background verified that STA1 is essential in expression of necessary genes including HSFA3 for two-step heat stress tolerance in plants. However, constitutive overexpression of the cDNA version of HSFA3 in the sta1-1 background is unable to execute plant heat stress tolerance in sta1-1. Consistently our global target analysis of STA1 showed that its splicing activity modulates a rather broad range of gene expression in response to heat treatment. The findings of this study reveal that heat-inducible STA1 activity for pre-mRNA splicing serves as a molecular regulatory mechanism underlying the plant stress tolerance to high-temperature stress.
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