Melatonin mitigates neomycin-induced hair cell injury in zebrafish
- Authors
- Oh, Kyoung Ho; Rah, Yoon Chan; Hwang, Kyu Ho; Lee, Seung Hoon; Kwon, Soon Young; Cha, Jae Hyung; Choi, June
- Issue Date
- 2017
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Melatonin; aminoglycoside; zebrafish; ototoxicity; protection
- Citation
- DRUG AND CHEMICAL TOXICOLOGY, v.40, no.4, pp.390 - 396
- Indexed
- SCIE
SCOPUS
- Journal Title
- DRUG AND CHEMICAL TOXICOLOGY
- Volume
- 40
- Number
- 4
- Start Page
- 390
- End Page
- 396
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86256
- DOI
- 10.1080/01480545.2016.1244679
- ISSN
- 0148-0545
- Abstract
- Context: Ototoxicity due to medications, such as aminoglycosides, is irreversible, and free radicals in the inner ear are assumed to play a major role. Because melatonin has an antioxidant property, we hypothesize that it might mitigate hair cell injury by aminoglycosides. Objective: The objective of this study was to evaluate whether melatonin has an alleviative effect on neomycin-induced hair cell injury in zebrafish (Danio rerio). Methods: Various concentrations of melatonin were administered to 5-day post-fertilization zebrafish treated with 125 mu M neomycin for 1 h. Surviving hair cells within four neuromasts were compared with that of a control group. Apoptosis was assessed via terminal deoxynucleotidyl transferase dUTP nick-end labeling assay. The changes of ultrastructure were confirmed using a scanning electron microscope. Results: Melatonin alleviated neomycin-induced hair cell injury in neuromasts (neomycin+melatonin 100 mu M: 13.88 +/- 0.91 cells, neomycin only: 7.85 +/- 0.90 cells; n=10, p<0.05) and reduced neomycin-induced apoptosis in the TUNEL assay. In ultrastructural analysis, hair cells within the neuromasts in zebrafish were preserved exposed to 125 mu M neomycin and 100 mu M melatonin for 1 h in SEM findings. Conclusion: Melatonin is effective in alleviating aminoglycoside-induced hair cell injury in zebrafish. The results of this study demonstrated that melatonin has the potential to reduce apoptosis induced by aminoglycosides in zebrafish.
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