Cactus cladodes (Opuntia humifusa) extract minimizes the effects of UV irradiation on keratinocytes and hairless mice
- Authors
- Park, Kyungmi; Choi, Hyeon-Son; Hong, Yang Hee; Jung, Eun Young; Suh, Hyung Joo
- Issue Date
- 2017
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Hyaluronic acid synthase; hyaluronidase; HA-binding protein; CD44; HaCaT cell; SKH-1 mice
- Citation
- PHARMACEUTICAL BIOLOGY, v.55, no.1, pp.1032 - 1040
- Indexed
- SCIE
SCOPUS
- Journal Title
- PHARMACEUTICAL BIOLOGY
- Volume
- 55
- Number
- 1
- Start Page
- 1032
- End Page
- 1040
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86394
- DOI
- 10.1080/13880209.2017.1286357
- ISSN
- 1388-0209
- Abstract
- Context: Cactus cladodes [Opuntia humifusa (Raf.) Raf. (Cactaceae)] is one of the cactus genera, which has long been used as a folk medicine for skin disorders. Objective: This study investigated the skincare potential of cactus cladodes extract (OHE), including its ability to regulate ultraviolet B (UVB)-induced hyaluronic acid (HA) production. Materials and methods: Gene expression levels of hyaluronic acid synthases (HASs) and hyaluronidase (HYAL) were measured in UVB-irradiated HaCaT cells with OHE treatment (10, 25, 50, 100 mu g/mL) by realtime polymerase chain reaction (PCR). The HA content was analyzed in hairless mice (SKH-1, male, 6 weeks old) treated with OHE for 10 weeks by using enzyme-linked immunosorbent assay (ELISA). Haematoxylin and eosin (H&E) and immunohistological staining were performed to examine epidermal thickness and levels of CD44 and hyaluronic acid-binding protein (HABP). Results: HA synthases (HAS, 1 HAS2, HAS3) mRNA levels were increased by 1.9-, 2.2- and 1.6-fold, respectively, with OHE treatment (100 mu g/mL), while UVB-induced increase of hyaluronidase mRNA significantly decreased by 35%. HA content in animal was decreased from 42.9 to 27.1 ng/mL by OHE treatment. HAS mRNA levels were decreased by 39%, but HYAL mRNA was increased by 50% in OHE group. CD44 and HABP levels, which were greatly increased by UVB-irradiation, were reduced by 64 and 60%, respectively. Epidermal thickness, transepidermal water loss (TEWL), and erythema formation was also decreased by 45 (45.7 to 24.2 mu m), 48 (48.8 to 25 g/h/m(2)) and 33%, respectively. Conclusion: OHE protects skin from UVB-induced skin degeneration in HaCaT cells and hairless mice.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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