Morphologic Changes of Zebrafish Melanophore after Intense Pulsed Light and Q-Switched Nd:YAG Laser Irradiation
- Authors
- Ryu, Hwa Jung; Lee, Ji Min; Jang, Hee Won; Park, Hae Chul; Rhyu, Im Joo; Kim, Il-Hwan
- Issue Date
- Dec-2016
- Publisher
- KOREAN DERMATOLOGICAL ASSOC
- Keywords
- Intense pulsed light; Melanophores; Zebrafish
- Citation
- ANNALS OF DERMATOLOGY, v.28, no.6, pp.711 - 717
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANNALS OF DERMATOLOGY
- Volume
- 28
- Number
- 6
- Start Page
- 711
- End Page
- 717
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86601
- DOI
- 10.5021/ad.2016.28.6.711
- ISSN
- 1013-9087
- Abstract
- Background: Recently, the pulse-in-pulse mode of intense pulsed light (IPL) has been used increasingly for the treatment of melasma. Objective: To observe the morphologic changes in the melanophore in adult zebrafish after irradiation with conventional and pulse-in-pulse IPL and Q-switched Nd:YAG (QSNY) laser. Methods: Adult zebrafish were irradiated with conventional and pulse-in-pulse mode of IPL. The conditions for conventional IPL were 3 mJ/cm(2), 560 nm filter, and pulse widths of 7, 20, and 35 msec. The pulse-in-pulse conditions were 3 mJ/cm(2) and on-time 1/off-time 2. The QSNY laser was used with the settings of 1,064 nm, 0.4J/cm(2), a 7 mm spot size, and one shot. Specimens were observed using a light microscope, a transmission electron microscope (TEM), a scanning electron microscope (SEM) and a confocal microscope. Results: After conventional IPL irradiation with a 7 msec pulse width, melanophore breakage was observed using light microscopy. Under TEM, irradiation with conventional IPL for 7 msec and pulse-in-pulse IPL induced melanophore thermolysis with vacuolization. However, changes in the melanophore were not observed with 35 msec IPL. Under SEM, unlike the control and QSNY groups, IPL-irradiated zebrafish showed finger -like fusion in the protein structure of scales. Specimens examined by a confocal microscope after conventional IPL irradiation showed a larger green-stained area on TUNEL staining than that after pulse-in-pulse mode IPL irradiation. Conclusion: Zebrafish irradiated with long pulse-IPL showed no morphologic changes using light microscopy, while morphological changes in melanophores were evident with use of TEM. Pulse-in-pulse mode IPL caused less damage than conventional IPL.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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