Effects of gangliosides from deer bone extract on the gene expressions of matrix metalloproteinases and collagen type II in interleukin-1 beta-induced osteoarthritic chondrocytes
- Authors
- Suh, Hyung Joo; Lee, Hyunji; Min, Byung Jung; Jung, Sung Ug; Jung, Eun Young
- Issue Date
- 12월-2016
- Publisher
- KOREAN NUTRITION SOC
- Keywords
- Chondrocytes; collagen type II; interleukin-1 beta; matrix metalloproteinases; osteoarthritis
- Citation
- NUTRITION RESEARCH AND PRACTICE, v.10, no.6, pp.569 - 574
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- NUTRITION RESEARCH AND PRACTICE
- Volume
- 10
- Number
- 6
- Start Page
- 569
- End Page
- 574
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86617
- DOI
- 10.4162/nrp.2016.10.6.569
- ISSN
- 1976-1457
- Abstract
- BACKGROUND/OBJECTIVES: We investigated the anti-osteoarthritic effects of deer bone extract on the gene expressions of matrix metalloproteinases (MMPs) and collagen type II (COL2) in interleukin-1 beta-induced osteoarthritis (OA) chondrocytes. MATERIALS/METHODS: Primary rabbit chondrocytes were treated as follows: CON (PBS treatment), NC (IL-1 beta treatment), PC (IL-1 beta + 100 mu g/mL glucosamine sulphate/chondroitin sulphate mixture), and DB (IL-1 beta + 100 mu g/mL. deer bone extract). RESULTS: The results of the cell viability assay indicated that deer bone extract at doses ranging from 100 to 500 mu g/mL inhibits cell death in chondrocytes induced by IL-1 beta. Deer bone extract was able to significantly recover the mRNA expression of COL2 that was down-regulated by IL-1 beta (NC: 0.79 vs. DB: 0.87, P < 0.05) and significantly decrease the mRNA expression of MMP-3 (NC: 2.24 vs. DB: 1.75) and -13 (NC: 1.28 vs. DB: 0.89) in OA chondrocytes (P < 0.05). CONCLUSIONS: We concluded that deer bone extract induces accumulation of COL2 through the down-regulation of MMPs in IL-1 beta-induced OA chondrocytes. Our results suggest that deer bone extract, which contains various components related to OA, including chondroitin sulphate, may possess anti-osteoarthritic properties and be of value in inhibiting the pathogenesis of OA.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.