Gr-1(int)CD11b(+) myeloid-derived suppressor cells accumulate in corneal allograft and improve corneal allograft survival
- Authors
- Choi, Wungrak; Ji, Yong Woo; Ham, Hwa-Yong; Yeo, Areum; Noh, Hyemi; Jin, Su-Eon; Song, Jong Suk; Kim, Hyeon Chang; Kim, Eung Kwon; Lee, Hyung Keun
- Issue Date
- 12월-2016
- Publisher
- WILEY
- Keywords
- keratoplasty; myeloid differentiation antigen Gr-1; interferon-gamma (IFN-gamma); TNF-related apoptosis-inducing ligand (TRAIL); adoptive transfer
- Citation
- JOURNAL OF LEUKOCYTE BIOLOGY, v.100, no.6, pp.1453 - 1463
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF LEUKOCYTE BIOLOGY
- Volume
- 100
- Number
- 6
- Start Page
- 1453
- End Page
- 1463
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86770
- DOI
- 10.1189/jlb.5A1115-508RR
- ISSN
- 0741-5400
- Abstract
- We identified the characteristics of myeloid-derived suppressor cells (MDSCs) and investigated their mechanism of induction and their functional role in allograft rejection using a murine corneal allograft model. In mice, MDSCs coexpress CD11b and myeloid differentiation antigen Gr-1.Gr-1(+)CD11b(+) cells infiltrated allografted corneas between 4 d and 4 wk after surgery; however, the frequencies of Gr-1(+)CD11b(+) cells were not different between accepted and rejected allografts or in peripheral blood or BM. Of interest, Gr-1(int)CD11b(+) cells, but not Gr-1(hi)CD11b(+) cells, infiltrated the accepted graft early after surgery and expressed high levels of immunosuppressive cytokines, including IL-10, TGF-beta, and TNF-related apoptosis-inducing ligand. This population remained until 4 wk after surgery. In vitro, only high dose (>100 ng/ml) of IFN-gamma plus GM-CSF could induce immunosuppressive cytokine expression in Gr-1(int)CD11b(+) cells. Furthermore, adoptive transfer of Gr-1(int)CD11b(+) cells reduced T cell infiltration, which improved graft survival. In conclusion, high-dose IFN-gamma in allograft areas is essential for development of Gr-1(int)CD11b(+) MDSCs in corneal allografts, and subtle environmental changes in the early period of the allograft can result in a large difference in graft survival.
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