Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen
- Authors
- Ha, Na-Young; Shin, Hyun Mu; Sharma, Prashant; Cho, Hyun Ah; Min, Chan-Ki; Kim, Hong-il; Nguyen Thi Hai Yen; Kang, Jae-Seung; Kim, Ik-Sang; Choi, Myung-Sik; Kim, Young Keun; Cho, Nam-Hyuk
- Issue Date
- 26-11월-2016
- Publisher
- BIOMED CENTRAL LTD
- Keywords
- Zinc oxide nanoparticle; ZnO binding peptide; Scrub typhus; Vaccine
- Citation
- JOURNAL OF NANOBIOTECHNOLOGY, v.14
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF NANOBIOTECHNOLOGY
- Volume
- 14
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86799
- DOI
- 10.1186/s12951-016-0229-2
- ISSN
- 1477-3155
- Abstract
- Background: Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP. Results: A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (K-a = 2.26 x 10(6) M-1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-gamma-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen. Conclusions: ZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system.
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Collections - College of Engineering > Department of Materials Science and Engineering > 1. Journal Articles
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