Detection of Silver Ions Using Dielectrophoretic Tweezers-Based Force Spectroscopy
- Authors
- Choi, Seungyeop; Lee, Gyudo; Park, In Soo; Son, Myeonggu; Kim, Woong; Lee, Hyungbeen; Lee, Sei-Young; Na, Sungsoo; Yoon, Dae Sung; Bashir, Rashid; Park, Jinsung; Lee, Sang Woo
- Issue Date
- 15-11월-2016
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Silver ion detection; dielectrophoresis; DEP tweezers
- Citation
- ANALYTICAL CHEMISTRY, v.88, no.22, pp.10867 - 10875
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 88
- Number
- 22
- Start Page
- 10867
- End Page
- 10875
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/86824
- DOI
- 10.1021/acs.analchem.6b00107
- ISSN
- 0003-2700
- Abstract
- Understanding of the interactions of silver ions (Ag+) with polynucleotides is important not only to detect Ag+ over a wide range of concentrations in a simple, robust, and high-throughput manner but also to investigate the intermolecular interactions of hydrogen and coordinate interactions that are generated due to the interplay of Ag+, hydrogen ions (H+), and polynucleotides since it is critical to prevent adverse environmental effects that may cause DNA damage and develop strategies to treat this damage. Here, we demonstrate a novel approach to simultaneously detect Ag+ satisfying the above requirements and examine the combined intermolecular interactions of Ag+-polycytosine and H+-polycytosine DNA complexes using dielectrophoretic tweezers-based force spectroscopy. For this investigation, we detected Ag+ over a range of concentrations (1 nM to 100 mu M) by quantifying the rupture force of the combined interactions and examined the interplay between the three factors (Ag+, H+, and polycytosine) using the same assay for the detection of Ag+. Our study provides a new avenue not only for the detection of heavy metal ions but also for the investigation of heavy metal ions-polynucleotide DNA complexes using the same assay.
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Collections - Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
- College of Engineering > Department of Mechanical Engineering > 1. Journal Articles
- Graduate School > Department of Bioengineering > 1. Journal Articles
- Graduate School > Department of Control and Instrumentation Engineering > 1. Journal Articles
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