Macrophage-Targeted Indocyanine Green-Neomannosyl Human Serum Albumin for Intraoperative Sentinel Lymph Node Mapping in Porcine Esophagus
- Authors
- Kim, Hyun Koo; Quan, Yu Hua; Oh, Yujin; Park, Ji Yong; Park, Ji-Ho; Choi, Yeonho; Lee, Yun-Sang; Jeong, Jae Min; Choi, Young Ho; Kim, Beop-Min
- Issue Date
- 10월-2016
- Publisher
- ELSEVIER SCIENCE INC
- Citation
- ANNALS OF THORACIC SURGERY, v.102, no.4, pp.1149 - 1155
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANNALS OF THORACIC SURGERY
- Volume
- 102
- Number
- 4
- Start Page
- 1149
- End Page
- 1155
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/87295
- DOI
- 10.1016/j.athoracsur.2016.04.077
- ISSN
- 0003-4975
- Abstract
- Background. The sentinel lymph node (SLN) concept has been proposed to avoid unnecessary invasive LN dissection in surgery for esophageal cancer. This study evaluated a new macrophage-targeting fluorescent agent, indocyanine green-neomannosyl human serum albumin (ICG: MSA), for SLN mapping using a custom-made intraoperative color and fluorescence-merged imaging system (ICFIS) in porcine esophagus. Methods. The LN targeting ability of ICG: MSA, indocyanine green-human serum albumin (ICG: HSA), and ICG was examined in vitro using the U937 differentiated monocyte cell line and in vivo in a mouse footpad model using fluorescence imaging. SLN identification in rabbit esophagus was then performed using ICG: MSA, ICG: HSA, and ICG. Finally, intraoperative SLN detection was conducted in porcine esophagus after esophagoscopic injection of ICG: MSA. Results. The fluorescence signal of U937 cells treated by ICG: MSA was significantly higher than that of ICG or ICG: HSA(ICG: 1.0 +/- 0.37; ICG: HSA: 3.4 +/- 0.28, ICG: MSA: 6.8 +/- 1.61; ICG to ICG: HSA, p = 0.03; ICG: HSA to ICG: MSA, p = 0.04; ICG to ICG: MSA, p = 0.0009). ICG: MSA was retained in popliteal LNs as long as 3 h, while ICG rapidly diffused through the entire mouse lymphatic system within 5 min. Esophageal SLN was detected within 15 min after injection of either ICG or ICG: MSA, but ICG: MSA provided more distinguishable images of LNss than ICG in rabbit esophagus. The SLN was also successfully detected in all porcine esophagus; the mean number of SLNs identified per esophagus was 1.6 +/- 0.55. Conclusions. ICG: MSA has more specific macrophage-targeting properties, which could overcome the limitation of the low SLN retention of ICG, and could provide more precise real-time SLN detection during esophageal cancer surgery.(C) 2016 by The Society of Thoracic Surgeons.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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