Surface plasmon resonance sensing of a biomarker of Alzheimer disease in an intensity measurement mode with a bimetallic chip
- Authors
- Kim, Hyung Jin; Sohn, Young-Soo; Kim, Chang-duk; Jang, Dae-ho
- Issue Date
- 9월-2016
- Publisher
- KOREAN PHYSICAL SOC
- Keywords
- Bimetallic chip; Surface plasmon resonance; Reflectance measurement; Amyloid-beta
- Citation
- JOURNAL OF THE KOREAN PHYSICAL SOCIETY, v.69, no.5, pp.793 - 797
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF THE KOREAN PHYSICAL SOCIETY
- Volume
- 69
- Number
- 5
- Start Page
- 793
- End Page
- 797
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/87614
- DOI
- 10.3938/jkps.69.793
- ISSN
- 0374-4884
- Abstract
- A surface plasmon resonance (SPR) sensor system with a bimetallic chip has been utilized to sense the very low concentration of amyloid-beta (A beta)(1-42) by measurement of the reflectance variation. The bimetallic chip was comprised of Au (10 nm) and Ag (40 nm) on Cr (2 nm)-coated BK-7 glass substrate. Protein A was used to efficiently immobilize the antibody of A beta(1-42) on the surface of the bimetallic chip. The reflectance curve of the bimetallic chip represented a narrower linewidth compared to that of the conventional gold (Au) chip. The SPR sensor using the bimetallic chip in the intensity interrogation mode acquired the response of A beta(1-42) at concentrations of 250, 500, 750 and 1,000 pg/ml. The calibration plot showed a linear relationship between the mean reflectance variation and the A beta(1-42) concentration. The results proved that the SPR sensor system with the bimetallic chip in the intensity interrogation mode can successfully detect various concentrations of A beta(1-42), including critical concentration, to help diagnose Alzheimer's disease.
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