Plantamajoside Inhibits UVB and Advanced Glycation End Products-Induced MMP-1 Expression by Suppressing the MAPK and NF-B Pathways in HaCaT Cells
- Authors
- Han, Ah-Ram; Nam, Mi-Hyun; Lee, Kwang-Won
- Issue Date
- 9월-2016
- Publisher
- WILEY-BLACKWELL
- Citation
- PHOTOCHEMISTRY AND PHOTOBIOLOGY, v.92, no.5, pp.708 - 719
- Indexed
- SCIE
SCOPUS
- Journal Title
- PHOTOCHEMISTRY AND PHOTOBIOLOGY
- Volume
- 92
- Number
- 5
- Start Page
- 708
- End Page
- 719
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/87718
- DOI
- 10.1111/php.12615
- ISSN
- 0031-8655
- Abstract
- Photoaging and glycation stress are major causes of skin deterioration. Oxidative stress caused by ultraviolet B (UVB) irradiation can upregulate matrix metalloprotease 1 (MMP-1), a major enzyme responsible for collagen damage in the skin. Advanced glycation end products (AGEs) accumulate via gradual formation from skin proteins, especially from long-lived proteins such as dermal elastin and collagen. Plantamajoside (PM), isolated from Plantago asiatica, has various biological effects including anti-inflammatory and antioxidant effects. In this study, we assessed the protective effects of PM on a human keratinocyte cell line (HaCaT) and primary human dermal fibroblasts (HDF) against stress caused by glyceraldehyde-induced AGEs (glycer-AGEs) with UVB irradiation. We found that PM attenuated UVB- and-glycer-AGEs-induced MMP-1 expression in HaCaT and HDF cells and proinflammatory cytokines expression by inhibiting the phosphorylation of mitogen-activated protein kinases (MAPKs) activated by reactive oxygen species. Specific inhibitors of NF-B and MAPKs attenuated the induced expression of MMP-1. PM also inhibited the phosphorylation of IB, and reduced nuclear translocation of NF-B in these cells. Furthermore, PM attenuated the upregulation of receptor for AGEs (RAGE) by glycer-AGEs with UVB irradiation. Therefore, our findings strongly suggest that PM is a promising inhibitor of skin photoaging.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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