Structural and immunological feature of rhamnogalacturonan I-rich polysaccharide from Korean persimmon vinegar
- Authors
- Kim, Hoon; Hong, Hee-Do; Suh, Hyung-Joo; Shin, Kwang-Soon
- Issue Date
- 8월-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Persimmon vinegar; Polysaccharide; Rhamnogalacturonan I; Mannan; Macrophage stimulation
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.89, pp.319 - 327
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 89
- Start Page
- 319
- End Page
- 327
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/87911
- DOI
- 10.1016/j.ijbiomac.2016.04.060
- ISSN
- 0141-8130
- Abstract
- The crude polysaccharide (KPV-0) isolated from Korean persimmon vinegar was fractionated using gel filtration chromatography to enhance the immunostimulatory activity and to identify the structural features of active fraction. Among three fractions, KPV-I obtained in a void volume, demonstrated the potent production of macrophage-stimulating mediators, including tumor necrosis factor-alpha, interleukin (IL)-6, IL-12, and nitric oxide. KPV-I showed a combined single peak with high molecular weight of 55,000 Da by high performance size exclusion chromatography. Component sugar analysis revealed that KPV-I contained mainly of arabinose, mannose, galactose, rhamnose and galacturonic acid. Single radial gel diffusion assay using beta-glucosyl Yariv reagent showed that KPV-I contained arabinogalactan protein with 13.7%. Methylation analysis indicated that KPV-I contained 21 kinds of neutral glycosidic linkages, which seemed to be composed three kinds of polysaccharide; that is a rhamnogalacturonan-I (65-70%) derived from persimmon as a raw material, a mannan (20-25%) derived from fermentation-associated microorganisms, and a linear glucans (less than 10%). In conclusion, polysaccharide isolated from persimmon vinegar could augment the macrophage stimulation, and a large amounts of RG-I polysaccharide derived from persimmon is likely a crucial role in expression of the activity in persimmon vinegar. (C) 2016 Elsevier B.V. All rights reserved.
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