Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

High-throughput and rapid quantification of lipids by nanoflow UPLC-ESI-MS/MS: application to the hepatic lipids of rabbits with nonalcoholic fatty liver disease

Authors
Byeon, Seul KeeLee, Jong CheolChung, Bong ChulSeo, Hong SeogMoon, Myeong Hee
Issue Date
7월-2016
Publisher
SPRINGER HEIDELBERG
Keywords
Hepatic lipid; Inflammation; High-cholesterol diet; Nonalcoholic fatty liver disease; nUPLC-ESI-MS/MS
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.408, no.18, pp.4975 - 4985
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume
408
Number
18
Start Page
4975
End Page
4985
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/88148
DOI
10.1007/s00216-016-9592-y
ISSN
1618-2642
Abstract
A rapid and high-throughput quantification method (approximately 300 lipids within 20 min) was established using nanoflow ultrahigh-pressure liquid chromatography-tandem mass spectrometry (nUPLC-ESI-MS/MS) with selective reaction monitoring (SRM) and applied to the quantitative profiling of the hepatic lipids of rabbits with different metabolic conditions that stimulate the development of nonalcoholic fatty liver disease (NAFLD). Among the metabolic conditions of rabbits in this study [inflammation (I), high-cholesterol diet (HC), and high-cholesterol diet combined with inflammation (HCI)], significant perturbation in hepatic lipidome (> 3-fold and p < 0.01) was observed in the HC and HCI groups, while no single lipid showed a significant change in group I. In addition, this study revealed a dramatic increase (> 2-fold) in relatively high-abundant monohexosylceramides (MHCs), sphingomyelins (SMs), and triacylglycerols (TGs) in both the HC and HCI groups, especially in MHCs as all 11 MHCs increased by larger than 3- to 12-fold. As the levels of the relatively high-abundant lipids in the above classes increased, the total lipidome level of each class increased significantly by approximately 2-fold to 5-fold. Other classes of lipids also generally increased, which was likely induced by the increase in mitogenic and nonapoptotic MHCs and SMs, as they promote cell proliferation. On the other hand, a slight decrease in the level of apoptotic ceramides (Cers) was observed, which agreed with the general increase in total lipid level. As distinct changes in hepatic lipidome were observed from HC groups, this suggests that HC or HCI is highly associated with NAFLD but not inflammation alone itself.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE