Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf
- Authors
- Kim, Hoon; Kwak, Bong-Shin; Hong, Hee-Do; Suh, Hyung-Joo; Shin, Kwang-Soon
- Issue Date
- 6월-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Barley leaf; Pectinase digestion; Immunostimulatory activity
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.87, pp.308 - 316
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 87
- Start Page
- 308
- End Page
- 316
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/88483
- DOI
- 10.1016/j.ijbiomac.2016.02.072
- ISSN
- 0141-8130
- Abstract
- Four polysaccharide fractions were isolated from young barley leaves treated with or without pectinase followed by ethanol fractionation. Among the polysaccharide fractions, BLE-P isolated from pectinase digested with a high molecular weight had the most enhanced macrophage stimulatory activity, indicating that pectinase digestion of barley leaf is a useful method for enhancement of its activity. BLE-P was further purified by column chromatography to identify the chemical and structural properties. BLEP-I eluted in void volume fraction showed potent macrophage stimulatory activity. Monosaccharide composition and linkage analysis indicated that at least three kinds of polysaccharide, that is, glucuronoarabinoxylan (GAX; 40-45%), rhamnogalacturonan-I (RG-I) with branching mainly involving a type II arabinogalactan (AG-II) side chain (30-35%), and linear glucan such as starch and cellulose (less than 10%) coexisted in BLE-P-I. Given the association with macrophage stimulatory activity, it is likely that the GAX and to the RG-I polysaccharide branched with an AG-II side chain may be important for expression of the activity in barley leaf. (C) 2016 Elsevier B.V. All rights reserved.
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