Deer Bone Oil Extract Suppresses Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hyeon-Son | - |
dc.contributor.author | Im, Suji | - |
dc.contributor.author | Park, Yooheon | - |
dc.contributor.author | Hong, Ki-Bae | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.date.accessioned | 2021-09-04T01:07:42Z | - |
dc.date.available | 2021-09-04T01:07:42Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2016-04 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/89089 | - |
dc.description.abstract | The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, alpha-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-mediated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1 beta, and IL-12 beta, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DSO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC JAPAN | - |
dc.subject | COLLAGEN-INDUCED ARTHRITIS | - |
dc.subject | RHEUMATOID-ARTHRITIS | - |
dc.subject | CERVUS-NIPPON | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | ANTLER | - |
dc.subject | OSTEOARTHRITIS | - |
dc.subject | MACROPHAGES | - |
dc.subject | CYTOKINE | - |
dc.subject | MICE | - |
dc.subject | CYCLOOXYGENASE | - |
dc.title | Deer Bone Oil Extract Suppresses Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1248/bpb.b15-00952 | - |
dc.identifier.scopusid | 2-s2.0-84964608605 | - |
dc.identifier.wosid | 000373246100017 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.39, no.4, pp.593 - 600 | - |
dc.relation.isPartOf | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.title | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.volume | 39 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 593 | - |
dc.citation.endPage | 600 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | COLLAGEN-INDUCED ARTHRITIS | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | CERVUS-NIPPON | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | ANTLER | - |
dc.subject.keywordPlus | OSTEOARTHRITIS | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | CYCLOOXYGENASE | - |
dc.subject.keywordAuthor | deer bone oil extract (DBOE) | - |
dc.subject.keywordAuthor | RAW264.7 | - |
dc.subject.keywordAuthor | inducible nitric oxide synthase (iNOS) | - |
dc.subject.keywordAuthor | nitric oxide (NO) | - |
dc.subject.keywordAuthor | anti-inflammatory response | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.