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Deer Bone Oil Extract Suppresses Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Cells

Authors
Choi, Hyeon-SonIm, SujiPark, YooheonHong, Ki-BaeSuh, Hyung Joo
Issue Date
4월-2016
Publisher
PHARMACEUTICAL SOC JAPAN
Keywords
deer bone oil extract (DBOE); RAW264.7; inducible nitric oxide synthase (iNOS); nitric oxide (NO); anti-inflammatory response
Citation
BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.39, no.4, pp.593 - 600
Indexed
SCIE
SCOPUS
Journal Title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume
39
Number
4
Start Page
593
End Page
600
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/89089
DOI
10.1248/bpb.b15-00952
ISSN
0918-6158
Abstract
The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, alpha-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-mediated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1 beta, and IL-12 beta, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DSO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.
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Suh, Hyung Joo
보건과학대학 (바이오시스템의과학부)
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