Effect of Titanium Dioxide Nanoparticle Exposure on the Ocular Surface: An Animal Study
- Authors
- Eom, Youngsub; Song, Jong Suk; Lee, Dong Yeol; Kim, Myo-Kyoung; Kang, Bo-Ram; Heo, Jeong-Hwa; Lee, Hyung Keun; Kim, Hyo Myung
- Issue Date
- 4월-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- ocular surface; nanoparticle; titanium dioxide
- Citation
- OCULAR SURFACE, v.14, no.2, pp.224 - 232
- Indexed
- SCIE
SCOPUS
- Journal Title
- OCULAR SURFACE
- Volume
- 14
- Number
- 2
- Start Page
- 224
- End Page
- 232
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89119
- DOI
- 10.1016/j.jtos.2015.12.003
- ISSN
- 1542-0124
- Abstract
- Purpose: To evaluate the effect of titanium dioxide (TiO2) nanoparticle exposure on the ocular surface. Methods: Eighty eyes of 40 rabbits were used. The TiO2-1D group (n = 20) received a single instillation of TiO2 in the right eye. The TiO2-4D group (n = 20) received a TiO2 instillation in the right eye once a day for four days. The 40 untreated left eyes were used as controls. Ocular surface staining (n = 5 for each group) was performed with rose bengal dye, tear secretion (n = 5) was measured using the phenol red thread test, lactic dehydrogenase (LDH) activity (n = 5) and MUC5AC levels (n = 5) were measured in tears, and the area of the conjunctival goblet cells (n = 5) was measured through impression cytology and scanning electron microscopy 24 hours after the last TiO2 instillation. Results: Ocular surface staining was increased but the tear secretion was not changed after TiO2 exposure. The TiO2-1D (1.39 OD) and TiO2-4D groups (0.58 OD) had higher median tear LDH activity than the control groups (0.57 OD and 0.29 OD, respectively). Although the median tear MUC5AC level in the TiO2-1D group (92.7 ng/ml) was higher than that of control 1 group (37.4 ng/ml), there was no significant difference in MUC5AC levels between the TiO2-4D and control 2 groups. Conjunctival goblet cell area decreased after TiO2 exposure. Conclusions: Exposure to TiO2 nanoparticles induced ocular surface damage. Although the tear MUC5AC level increased after a single exposure, it decreased to normal levels after repeated exposures. The area of conjunctival goblet cells decreased after TiO2 exposure.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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