Simple and Biocompatible Ion Beam Micropatterning of a Cell-Repellent Polymer on Cell-Adhesive Surfaces to Manipulate Cell Adhesion
- Authors
- Hwang, In-Tae; Jung, Chan-Hee; Jung, Chang-Hee; Choi, Jae-Hak; Shin, Kwanwoo; Yoo, Young-Do
- Issue Date
- 2월-2016
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- PNIPAAm; Cell Micropatterns; Ion Irradiation; Tissue Culture Polystyrene; Protein Adsorption
- Citation
- JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.12, no.2, pp.387 - 393
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
- Volume
- 12
- Number
- 2
- Start Page
- 387
- End Page
- 393
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89608
- DOI
- 10.1166/jbn.2016.2184
- ISSN
- 1550-7033
- Abstract
- In this paper, the simple and biocompatible micropatterning of cell-repellent poly(N-isopropylacrylamide) (PNIPAAm) on a cell-adhesive substrate by ion beam micropatterning to control cell adhesion is described. Cell-repellent PNIPAAm films spin-coated on cell-adhesive tissue culture polystyrene (TCPS) substrates were selectively irradiated by energetic proton ions at various fluences through a pattern mask, and subsequently developed to create the micropatterns of PNIPAAm. Well-defined negative-type PNIPAAm micropatterns were successfully created on the TCPS substrates at fluences higher than 5 x 10(14) ions/cm(2), and their chemical properties were dependent on the fluence. Moreover, based on the results of the protein adsorption and in-vitro cell culture tests, 200 mu m well-defined micropatterns of mammalian cells were clearly formed on the PNIPAAm-micropatterned TCPS substrates though the preferential adsorption and growth of cells on the TCPS regions due to the strong cell-repellency of PNIPAAm.
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