Properties of a glycogen like polysaccharide produced by a mutant of Escherichia coli lacking glycogen synthase and maltodextrin phosphorylase
DC Field | Value | Language |
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dc.contributor.author | Kwak, Ji-Yun | - |
dc.contributor.author | Kim, Min-Gyu | - |
dc.contributor.author | Kim, Young-Wan | - |
dc.contributor.author | Ban, Hyun-Seung | - |
dc.contributor.author | Won, Mi-Sun | - |
dc.contributor.author | Park, Jong-Tae | - |
dc.contributor.author | Park, Kwan-Hwa | - |
dc.date.accessioned | 2021-09-04T03:44:51Z | - |
dc.date.available | 2021-09-04T03:44:51Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2016-01-20 | - |
dc.identifier.issn | 0144-8617 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/89781 | - |
dc.description.abstract | Escherichia coli mutant TBP38 lacks glycogen synthase (GlgA) and maltodextrin phosphorylase (MalP). When grown on maltose in fed-batch fermentation TBP38 accumulated more than 50-fold higher glycogen-type polysaccharide than its parental strain. The polysaccharides were extracted at different growth stages and migrated as one peak in size-exclusion chromatography. TBP38 produced polysaccharides ranging 2.6 x 10(6)-4.6 x 10(6) Da. A ratio of short side-chains (DP <= 12) in the polysaccharides was greater than 50%, and number-average degree of polymerization varied from 9.8 to 8.4. The polysaccharides showed 70-290 times greater water-solubility than amylopectin. K-m values using porcine and human pancreatic alpha-amylases with polysaccharides were 2- to 4-fold larger than that of amylopectin. k(cat) values were similar for both alpha-amylases. The TBP38 polysaccharides had 40-60% lower digestibility to amyloglucosidase than amylopectin. Intriguingly, the polysaccharides showed strong immunostimulating effects on mouse macrophage cell comparable to lipopolysaccharides. The lipopolysaccharide contamination levels were too low to account for this effect. (C) 2015 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | BRANCHING ENZYME | - |
dc.subject | STARCH | - |
dc.title | Properties of a glycogen like polysaccharide produced by a mutant of Escherichia coli lacking glycogen synthase and maltodextrin phosphorylase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Wan | - |
dc.identifier.doi | 10.1016/j.carbpol.2015.09.091 | - |
dc.identifier.scopusid | 2-s2.0-84943563471 | - |
dc.identifier.wosid | 000365972000076 | - |
dc.identifier.bibliographicCitation | CARBOHYDRATE POLYMERS, v.136, pp.649 - 655 | - |
dc.relation.isPartOf | CARBOHYDRATE POLYMERS | - |
dc.citation.title | CARBOHYDRATE POLYMERS | - |
dc.citation.volume | 136 | - |
dc.citation.startPage | 649 | - |
dc.citation.endPage | 655 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | BRANCHING ENZYME | - |
dc.subject.keywordPlus | STARCH | - |
dc.subject.keywordAuthor | Escherichia coli mutant | - |
dc.subject.keywordAuthor | Intracellular polysaccharide | - |
dc.subject.keywordAuthor | Glycogen | - |
dc.subject.keywordAuthor | Amylase | - |
dc.subject.keywordAuthor | Digestibility kinetics | - |
dc.subject.keywordAuthor | Water solubility | - |
dc.subject.keywordAuthor | Immunostimulating effect | - |
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