Pheophorbide a from Capsosiphon fulvescens Inhibits Advanced Glycation End Products Mediated Endothelial Dysfunction
- Authors
- Hong, Chung-Oui; Nam, Mi-Hyun; Oh, Ji-Sun; Lee, Jin-Won; Kim, Cheong-Tae; Park, Kwon-Woo; Lee, Dong-Ho; Lee, Kwang-Won
- Issue Date
- 1월-2016
- Publisher
- GEORG THIEME VERLAG KG
- Keywords
- Capsosiphon fulvescens; Capsosiphonaceae; pheophorbide a; advanced glycation end product; proinflammatory cytokine; endothelial cell dysfunction
- Citation
- PLANTA MEDICA, v.82, no.1-2, pp.46 - 57
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLANTA MEDICA
- Volume
- 82
- Number
- 1-2
- Start Page
- 46
- End Page
- 57
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89968
- DOI
- 10.1055/s-0035-1557829
- ISSN
- 0032-0943
- Abstract
- During hyperglycemia, the first step toward the formation of advanced glycation end products is the nonenzymatic glycation between the carbonyl group of a sugar and the primary amino group of a protein. Advanced glycation end products are then produced through more complex reactions. Reactive oxygen species derived from advanced glycation end products may play a key role in inflammation of the endothelium, leading to the complications seen in diabetes. Glycolaldehyde-induced advanced glycation end products have been reported to express proinflammatory cytokines, such as tumor necrosis factor-a and interleukin-1 beta. This study focused on Capsosiphon fulvescens, a Capsosiphonaceae type of green algae that has shown potential as a functional food material. Pheophorbide a, an anti-glycation compound, was isolated from C. fulvescens by extraction using a mixture of ethanol and water, followed by column fractionation of the resulting extract. The compound separated from C. fulvescens was identified by means of high-performance liquid chromatography combined with mass spectrometry. Pheophorbide a showed scavenging activity of the intracellular reactive oxygen species as well as monocyte adhesiveness inhibitory activity on the human myelomonocytic cell line (THP-1) and human umbilical vein endothelial cells cocultivation system. The mRNA levels of inflammation-related genes such as monocyte chemoattractant protein-1 and interleukin-6 were significantly decreased by pheophorbide a, and advanced glycation end products-stimulated tumor necrosis factor-a and interleukin-1 beta were downregulated as well. These results indicate that pheophorbide a has significant reactive oxygen species-scavenging activity, monocyte adhesive inhibitory activity, and downregulatory activity of cytokines related to inflammation affecting the endothelium. Pheophorbide a could therefore be a promising candidate for modulating endothelial cell dysfunction.
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Collections - Graduate School > Department of Plant Biotechnology > 1. Journal Articles
- Graduate School > Department of Biotechnology > 1. Journal Articles
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