Topical application of spent coffee ground extracts protects skin from ultraviolet B-induced photoaging in hairless mice
- Authors
- Choi, Hyeon-Son; Park, Eu Ddeum; Park, Yooheon; Han, Sung Hee; Hong, Ki Bae; Suh, Hyung Joo
- Issue Date
- 2016
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v.15, no.6, pp.779 - 790
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
- Volume
- 15
- Number
- 6
- Start Page
- 779
- End Page
- 790
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/90157
- DOI
- 10.1039/c6pp00045b
- ISSN
- 1474-905X
- Abstract
- The aim of this study was to evaluate the protective effect of spent coffee ground (SCG) on ultraviolet (UV) B-induced photoaging in hairless mice. The oil fraction (OSCG) and ethanol extract (ESCG) of SCG were prepared from SCG. OSCG contained a much higher level of caffeine (547.32 +/- 1.68 mu g mg(-1)) when compared to the sum of its chlorogenic acid derivatives (similar to 119 mu g mg(-1)), and pyrazines were the major aromatic compounds in OSCG. OSCG effectively inhibited the UVB-induced increase in intracellular reactive oxygen species in HaCaT cells. Topical application of OSCG or ESCG significantly reduced the UVB-induced wrinkle formation in mice dorsal skin. The combined application of OSCG and ESCG (OEH) led to a decrease in the wrinkle area by over 35% when compared with the UVB-treated control (UVBC). Epidermal thickness was also reduced by 40%. This result was connected to the significant reduction in transdermal water loss (27%) and erythema formation (48%) that result from UVB irradiation. Polarizationsensitive optical coherence tomography (PS-OCT) and antibody-based histological analyses showed that OSCG and ESCG effectively suppressed the UVB-induced decrease in collagen content. The level of type 1 collagen (COL1) in the OEH group was enhanced by around 40% compared with the UVB control group (UVBC). This was attributed to the down-regulation of matrix metalloproteinases (MMP2, 9, and 13), which are known to be responsible for collagen destruction. Our results indicate that topical treatment with OSCG/ESCG protects mouse skin from UVB-induced photoaging by down-regulating MMPs; therefore, suggesting the potential of SCG extracts as a topical anti-photoaging agent.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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