Anti-adipogenic effect of oat hull extract containing tricin on 3T3-L1 adipocytes
- Authors
- Lee, Dabeen; Park, Hyeong Yeol; Kim, Sohyun; Park, Yooheon; Bang, Myun-Ho; Imm, Jee-Young
- Issue Date
- 12월-2015
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Oat hull extract; 3T3-L1 adipocytes; Tricin; Adipogenesis; Transcription factors
- Citation
- PROCESS BIOCHEMISTRY, v.50, no.12, pp.2314 - 2321
- Indexed
- SCIE
SCOPUS
- Journal Title
- PROCESS BIOCHEMISTRY
- Volume
- 50
- Number
- 12
- Start Page
- 2314
- End Page
- 2321
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/91788
- DOI
- 10.1016/j.procbio.2015.09.019
- ISSN
- 1359-5113
- Abstract
- Oat hull ethyl acetate extract (50 mu g/mu L) significantly inhibited triglyceride accumulation in 3T3-L1 adipocytes without causing cytotoxicity. The major active compound in the extract was isolated and identified as 5,7,4'-trihydroxy-3',5'-dimethoxyflavone, tricin by mass spectrometry and 1H and C-13 NMR analyses. The tricin content in oat hull was approximately 18 mg/kg dry weight, and tricib constituted 9.6% of the total phenolic compounds in oat hull extract. The treatment of 3T3-L1 cells either with extract or purified tricin resulted in decreased lipid accumulation. The extract significantly decreased the activity of glycerol-3-phosphate dyhydrogenase, a marker of adipocyte differentiation in a dose dependent manner. The expression of major transcription factors, such as peroxisome proliferator-activated receptor (PPAR)-gamma, CCATT/enhancer binding protein-alpha (CEBP/alpha), sterol regulatory binding protein 1 (SREBP1) and fatty acid synthase (FAS) was also significantly suppressed by the extract. When commercial purified tricin was added to 3T3-L1 cells the expression of all transcription factors were significantly decreased as low as 1.5 mu g/mu L treatment. These results suggest that oat hull extract containing tricin possesses anti-adipogeneic effect. (C) 2015 Elsevier Ltd. All rights reserved.
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