IL-33 induces Egr-1-dependent TSLP expression via the MAPK pathways in human keratinocytes
- Authors
- Ryu, Woo-In; Lee, Hana; Kim, Jin Hee; Bae, Hyun Cheol; Ryu, Hwa Jung; Son, Sang Wook
- Issue Date
- 11월-2015
- Publisher
- WILEY
- Keywords
- atopic dermatitis; early growth response protein 1; interleukin-33; mitogen-activated protein kinase; thymic stromal lymphopoietin
- Citation
- EXPERIMENTAL DERMATOLOGY, v.24, no.11, pp.857 - 863
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL DERMATOLOGY
- Volume
- 24
- Number
- 11
- Start Page
- 857
- End Page
- 863
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/91991
- DOI
- 10.1111/exd.12788
- ISSN
- 0906-6705
- Abstract
- Atopic dermatitis (AD) is a chronic inflammatory skin disease in which T-helper type 2 (Th2)-type immune responses are dominant. Th2 cytokine, interleukin (IL)-33 and thymic stromal lymphopoietin (TSLP) have been suggested to have an important role in AD. IL-33 is highly expressed in AD, but its role in AD has not yet been fully understood. To further identify the role of IL-33 in AD, we investigated the expression of TSLP induced by IL-33 in keratinocytes. This study revealed that IL-33 induced TSLP expression in human keratinocytes. Early growth response protein 1 (Egr)-1, which is an inflammatory transcriptional factor, is induced by IL-33. IL-33-mediated TSLP induction in keratinocytes was suppressed by treatment with mitogen-activated protein kinase (MAPK) inhibitors or small interfering RNA against Egr-1. Chromatin immunoprecipitation (ChIP) assay indicated the direct involvement of Egr-1 in IL-33-mediated TSLP induction. Taken together, these findings indicate that IL-33 may increase TSLP expression through an Egr-1-dependent mechanism via ERK1/2, JNK and p38 activation in keratinocytes. These data suggest that the IL-33-ERK/JNK/p38/Egr-1/TSLP axis is involved in allergic skin Th2 inflammation, and it may be a novel therapeutic target.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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