Irisin, a Novel Myokine, Regulates Glucose Uptake in Skeletal Muscle Cells via AMPK
- Authors
- Lee, Hye Jeong; Lee, Jung Ok; Kim, Nami; Kim, Joong Kwan; Kim, Hyung Ip; Lee, Yong Woo; Kim, Su Jin; Choi, Jong-Il; Oh, Yoonji; Kim, Jeong Hyun; Suyeon-Hwang; Park, Sun Hwa; Kim, Hyeon Soo
- Issue Date
- 6월-2015
- Publisher
- ENDOCRINE SOC
- Citation
- MOLECULAR ENDOCRINOLOGY, v.29, no.6, pp.873 - 881
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR ENDOCRINOLOGY
- Volume
- 29
- Number
- 6
- Start Page
- 873
- End Page
- 881
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/93380
- DOI
- 10.1210/me.2014-1353
- ISSN
- 0888-8809
- Abstract
- Irisin is a novel myokine produced by skeletal muscle. However, its metabolic role is poorly understood. In the present study, irisin induced glucose uptake in differentiated skeletal muscle cells. It increased AMP-activated protein kinase (AMPK) phosphorylation and the inhibition of AMPK blocked glucose uptake. It also increased reactive oxygen species (ROS) generation. N-acetyl cysteine, a ROS scavenger, blocked irisin-induced AMPK phosphorylation. Moreover, irisin activated p38 MAPK in an AMPK-dependent manner. The inhibition and knockdown of p38 MAPK blocked irisin-induced glucose uptake. A colorimetric absorbance assay showed that irisin stimulated the translocation of glucose transporter type 4 to the plasma membrane and that this effect was suppressed in cells pretreated with a p38 MAPK inhibitor or p38 MAPK small interfering RNA. In primary cultured myoblast cells, irisin increased the concentration of intracellular calcium. STO-609, a calcium/calmodulin-dependent protein kinase kinase inhibitor, blocked irisin-induced AMPK phosphorylation, implying that calcium is involved in irisin-mediated signaling. Our results suggest that irisin plays an important role in glucose metabolism via the ROS-mediated AMPK pathway in skeletal muscle cells.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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