Enzymatic synthesis of 3-O-alpha-maltosyl-L-ascorbate using an engineered cyclodextrin glucanotransferase
DC Field | Value | Language |
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dc.contributor.author | Ahn, Hee-Jeong | - |
dc.contributor.author | Li, Chao | - |
dc.contributor.author | Cho, Hye-Bin | - |
dc.contributor.author | Park, Sunghoon | - |
dc.contributor.author | Chang, Pahn-Shick | - |
dc.contributor.author | Kim, Young-Wan | - |
dc.date.accessioned | 2021-09-04T19:12:36Z | - |
dc.date.available | 2021-09-04T19:12:36Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015-02-15 | - |
dc.identifier.issn | 0308-8146 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/94397 | - |
dc.description.abstract | A mutant derived from a cyclodextrin glucantransferase with an alanine residue as its acid/base catalyst residue (CGT-E284A) catalyzed regioselective glycosylation at 3-OH of L-ascorbic acid using a-maltosyl fluoride (alpha G2F) and L-ascorbic acid as the donor and acceptor, respectively, yielding 3-O-alpha-maltosyl-L-ascorbate (AA3 alpha G2). The optimum conditions were determined by high-performance liquid chromatography analysis with 20 mM alpha G2F and 40 mM L-ascorbic acid as the substrates at pH 7.5 and 25 degrees C with 1 mg/ml of the enzyme for 24 h. Calcium ions bound in CGT-E284A played an important role in the transglycosylation. CGT-E284A exhibited typical saturation kinetic behaviour for aG2F at a fixed acceptor concentration (40 mM), and substrate inhibition by L-ascorbic acid was observed at high L-ascorbic acid concentrations (>60 mM). AA3 alpha G2 was isolated from a preparative scale reaction with a yield of 29%, and it showed extremely high stability under oxidative conditions. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | L-ASCORBIC-ACID | - |
dc.subject | NUCLEAR-MAGNETIC-RESONANCE | - |
dc.subject | THIOGLYCOSIDE SYNTHESIS | - |
dc.subject | MUTANT GLYCOSIDASES | - |
dc.subject | VITAMIN-C | - |
dc.subject | THIOGLYCOLIGASE | - |
dc.subject | STABILITY | - |
dc.subject | GLYCOSYLTRANSFERASE | - |
dc.subject | TRANSGLYCOSYLATION | - |
dc.subject | TRANSGLUCOSYLATION | - |
dc.title | Enzymatic synthesis of 3-O-alpha-maltosyl-L-ascorbate using an engineered cyclodextrin glucanotransferase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Wan | - |
dc.identifier.doi | 10.1016/j.foodchem.2014.07.110 | - |
dc.identifier.scopusid | 2-s2.0-84906660665 | - |
dc.identifier.wosid | 000343339800050 | - |
dc.identifier.bibliographicCitation | FOOD CHEMISTRY, v.169, pp.366 - 371 | - |
dc.relation.isPartOf | FOOD CHEMISTRY | - |
dc.citation.title | FOOD CHEMISTRY | - |
dc.citation.volume | 169 | - |
dc.citation.startPage | 366 | - |
dc.citation.endPage | 371 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.subject.keywordPlus | L-ASCORBIC-ACID | - |
dc.subject.keywordPlus | NUCLEAR-MAGNETIC-RESONANCE | - |
dc.subject.keywordPlus | THIOGLYCOSIDE SYNTHESIS | - |
dc.subject.keywordPlus | MUTANT GLYCOSIDASES | - |
dc.subject.keywordPlus | VITAMIN-C | - |
dc.subject.keywordPlus | THIOGLYCOLIGASE | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | GLYCOSYLTRANSFERASE | - |
dc.subject.keywordPlus | TRANSGLYCOSYLATION | - |
dc.subject.keywordPlus | TRANSGLUCOSYLATION | - |
dc.subject.keywordAuthor | L-Ascorbic acid | - |
dc.subject.keywordAuthor | Cyclodextrin glucanotransferase | - |
dc.subject.keywordAuthor | Acid/base mutant | - |
dc.subject.keywordAuthor | 3-O-alpha-Maltosyl-L-ascorbate | - |
dc.subject.keywordAuthor | Transglycosylation | - |
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