Expression of astrocyte elevated gene-1 (AEG-1) in human meningiomas and its roles in cell proliferation and survival
- Authors
- Park, Kyung-Jae; Yu, Mi Ok; Song, Na-Hyun; Kong, Doo-Sik; Park, Dong-Hyuk; Chae, Yang-Seok; Chung, Yong-Gu; Kang, Shin-Hyuk
- Issue Date
- 1월-2015
- Publisher
- SPRINGER
- Keywords
- Meningioma; Astrocyte elevated gene-1; Progression; Apoptosis
- Citation
- JOURNAL OF NEURO-ONCOLOGY, v.121, no.1, pp.31 - 39
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF NEURO-ONCOLOGY
- Volume
- 121
- Number
- 1
- Start Page
- 31
- End Page
- 39
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/94792
- DOI
- 10.1007/s11060-014-1603-2
- ISSN
- 0167-594X
- Abstract
- Astrocyte elevated gene-1 (AEG-1) has recently been proposed to be involved in tumor development, invasion, and metastasis in several human cancers. However, the functional importance of AEG-1 expression in human meningioma has not been determined. We investigate the level of AEG-1 expression by quantitative reverse transcription PCR, immunohistochemistry analysis, and western blotting in various human meningioma tissues and cells. To determine the suppressive effect of AEG-1 on meningioma progression, we inhibited AEG-1 expression using small interfering RNA and examined cell proliferation, apoptosis, colony formation and tumorigenicity in a mouse xenograft model. AEG-1 expression was frequently elevated at both mRNA and protein levels in meningioma tumor tissues and in meningioma-derived cells as well. This elevation was more commonly observed in high-grade tumors than in benign ones. The knockdown of AEG-1 led to a decrease in overall cell proliferation, as well as anchorage-independent growth of malignant meningioma. In addition, apoptotic cell death occurred in AEG-1 depleted meningioma cells through p-Akt and Bcl-2 suppression. Furthermore, a mouse xenograft meningioma model showed that inhibition of AEG-1 expression significantly decreased tumor growth. Altogether, these data show that the elevation of AEG-1 contributes to the malignant progression of meningiomas, suggesting that AEG-1 could be a novel therapeutic target against human meningiomas.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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