Protective effects of germinated and fermented soybean extract against tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells and in rats
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eun Young | - |
dc.contributor.author | Hong, Ki-Bae | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.contributor.author | Choi, Hyeon-Son | - |
dc.date.accessioned | 2021-09-05T00:48:41Z | - |
dc.date.available | 2021-09-05T00:48:41Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 2042-650X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96150 | - |
dc.description.abstract | The aim of the current study is to investigate the antioxidant and hepatoprotective effects of germinated and fermented soybean extract (GFSE) on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in HepG2 cells and in the rat liver. High performance liquid chromatography (HPLC) analysis showed that genistin (3.40 +/- 0.14 mu g mg(-1)) was the most abundant isoflavone in the GFSE. Coumestrol (1.00 +/- 0.04 mu g mg(-1)), daidzin (0.78 +/- 0.14 mu g mg(-1)), genistein (0.68 +/- 0.05 mu g mg(-1)), glycitin (0.54 +/- 0.02 mu g mg(-1)), glycitein (0.41 +/- 0.02 mu g mg(-1)), and daidzein (0.02 +/- 0.0 g mg(-1)) are also contained in decreasing order of content. GFSE significantly inhibited t-BHP-induced reactive oxygen species (ROS) production in HepG2 cells. This GFSE-induced ROS reduction was associated with the down-regulation of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), a pro-oxidant enzyme, and the up-regulation of the mRNA levels of antioxidant enzymes, including catalase, superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (Gpx) in the rat liver. In addition, increased levels of antioxidant enzyme mRNAs correlated with the enhanced enzymatic activities of SOD, catalase, and glutathione-S-transferase (GST). The antioxidant effect of GFSE was supported by the reduction in the levels of malondialdehyde (MDA), a hydroperoxide, and the serum levels of lactate dehydrogenase (LDH), a biomarker of cell damage, were also lowered by GFSE. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST), which are clinical biomarkers of liver function, were shown to be improved with GFSE administration. The effects of GFSE were attributable to an improvement in liver tissue morphology. Taken together, GFSE protected the liver from t-BHP-induced oxidative stress by regulating ROS-related enzymes. Our results suggest that GFSE might be a hepatoprotective source against oxidative stress. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | ANTIOXIDANT ACTIVITY | - |
dc.subject | FREE-RADICALS | - |
dc.subject | ISOFLAVONES | - |
dc.subject | MECHANISMS | - |
dc.subject | DAMAGE | - |
dc.subject | GLYCEOLLINS | - |
dc.subject | INHIBITION | - |
dc.subject | COMPONENTS | - |
dc.subject | APOPTOSIS | - |
dc.title | Protective effects of germinated and fermented soybean extract against tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells and in rats | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1039/c5fo00785b | - |
dc.identifier.scopusid | 2-s2.0-84946566739 | - |
dc.identifier.wosid | 000364821800012 | - |
dc.identifier.bibliographicCitation | FOOD & FUNCTION, v.6, no.11, pp.3512 - 3521 | - |
dc.relation.isPartOf | FOOD & FUNCTION | - |
dc.citation.title | FOOD & FUNCTION | - |
dc.citation.volume | 6 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 3512 | - |
dc.citation.endPage | 3521 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | FREE-RADICALS | - |
dc.subject.keywordPlus | ISOFLAVONES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | GLYCEOLLINS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | COMPONENTS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.