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Protective effects of germinated and fermented soybean extract against tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells and in rats

Authors
Kim, Eun YoungHong, Ki-BaeSuh, Hyung JooChoi, Hyeon-Son
Issue Date
2015
Publisher
ROYAL SOC CHEMISTRY
Citation
FOOD & FUNCTION, v.6, no.11, pp.3512 - 3521
Indexed
SCIE
SCOPUS
Journal Title
FOOD & FUNCTION
Volume
6
Number
11
Start Page
3512
End Page
3521
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/96150
DOI
10.1039/c5fo00785b
ISSN
2042-650X
Abstract
The aim of the current study is to investigate the antioxidant and hepatoprotective effects of germinated and fermented soybean extract (GFSE) on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in HepG2 cells and in the rat liver. High performance liquid chromatography (HPLC) analysis showed that genistin (3.40 +/- 0.14 mu g mg(-1)) was the most abundant isoflavone in the GFSE. Coumestrol (1.00 +/- 0.04 mu g mg(-1)), daidzin (0.78 +/- 0.14 mu g mg(-1)), genistein (0.68 +/- 0.05 mu g mg(-1)), glycitin (0.54 +/- 0.02 mu g mg(-1)), glycitein (0.41 +/- 0.02 mu g mg(-1)), and daidzein (0.02 +/- 0.0 g mg(-1)) are also contained in decreasing order of content. GFSE significantly inhibited t-BHP-induced reactive oxygen species (ROS) production in HepG2 cells. This GFSE-induced ROS reduction was associated with the down-regulation of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), a pro-oxidant enzyme, and the up-regulation of the mRNA levels of antioxidant enzymes, including catalase, superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (Gpx) in the rat liver. In addition, increased levels of antioxidant enzyme mRNAs correlated with the enhanced enzymatic activities of SOD, catalase, and glutathione-S-transferase (GST). The antioxidant effect of GFSE was supported by the reduction in the levels of malondialdehyde (MDA), a hydroperoxide, and the serum levels of lactate dehydrogenase (LDH), a biomarker of cell damage, were also lowered by GFSE. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST), which are clinical biomarkers of liver function, were shown to be improved with GFSE administration. The effects of GFSE were attributable to an improvement in liver tissue morphology. Taken together, GFSE protected the liver from t-BHP-induced oxidative stress by regulating ROS-related enzymes. Our results suggest that GFSE might be a hepatoprotective source against oxidative stress.
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Suh, Hyung Joo
보건과학대학 (바이오시스템의과학부)
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