Lipase-Catalysed Enrichment of gamma-Linolenic Acid from Evening Primrose Oil in a Solvent-Free System
- Authors
- Baeza-Jimenez, Ramiro; No, Da Som; Otero, Christina; Garcia, Hugo S.; Lee, Jeom Sig; Kim, In-Hwan
- Issue Date
- 7월-2014
- Publisher
- SPRINGER
- Keywords
- 1-Butanol; Candida rugosa lipase; Enrichment; Esterification; Evening primrose oil; gamma-Linolenic acid (GLA)
- Citation
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v.91, no.7, pp.1147 - 1153
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
- Volume
- 91
- Number
- 7
- Start Page
- 1147
- End Page
- 1153
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98030
- DOI
- 10.1007/s11746-014-2463-9
- ISSN
- 0003-021X
- Abstract
- The enrichment of gamma-linolenic acid (GLA) was carried out in a solvent-free system by lipase-catalysed esterification of free fatty acids from evening primrose oil (EPO-FA) and 1-butanol (BtOH). The lipase employed to conduct this study was a free preparation of Candida rugosa. Variables evaluated were: substrate molar ratio (1:4, 1:6, 1:8, 1:10 and 1:12, EPO-FA:BtOH), temperature (10, 20, 30, 40, 50 and 60 A degrees C), and enzyme loading (5, 10, 15 and 20 %, based on the total weight of substrates). GLA was highly enriched in the non-esterified fatty acid fraction since C. rugosa showed very low selectivity for this fatty acid. We were able to increase the content of GLA to ca. 70 wt.% under the following optimal conditions: 30 A degrees C, 10 % enzyme loading and a 1:10 molar ratio (EPO-FA:BtOH), after 24 h. An additional set of experiments was conducted whereby the amount of water was controlled by addition of molecular sieves to the reaction mixture. The latter experiments produced a higher GLA concentrate (83.74 wt.%), under the optimal conditions described above and by adding 10 % molecular sieves (based on the total weight of substrates) after 36 h.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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