Identification of Lactoferrin as a Human Dedifferentiation Factor Through the Studies of Reptile Tissue Regeneration Mechanisms
- Authors
- Bae, Kil Soo; Kim, Sun Young; Park, Soon Yong; Jeong, Ae Jin; Lee, Hyun Hee; Lee, Jungwoon; Cho, Yee Sook; Leem, Sun-Hee; Kang, Tae-Hong; Bae, Kwang-Hee; Kim, Jae Ho; Jung, Yong Woo; Jun, Woojin; Yoon, Suk Ran; Lee, Sang-Chul; Chung, Jin Woong
- Issue Date
- 6월-2014
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Dedifferentiation; iPSCs; lactoferrin; stem cell; tissue regeneration
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.24, no.6, pp.869 - 878
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 24
- Number
- 6
- Start Page
- 869
- End Page
- 878
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98276
- DOI
- 10.4014/jmb.1402.02009
- ISSN
- 1017-7825
- Abstract
- In this study, we performed two-dimensional electrophoresis with protein extracts from lizard tails, and analyzed the protein expression profiles during the tissue regeneration to identify the dedifferentiation factor. As a result, we identified 18 protein spots among total of 292 spots, of which proteins were specifically expressed during blastema formation. We selected lactoferrin as a candidate because it is the mammalian homolog of leech-derived tryptase inhibitor, which showed the highest frequency among the 18 proteins. Lactoferrin was specifically expressed in various stem cell lines, and enhanced the efficiency of iPSC generation upto approximately 7-fold relative to the control. Furthermore, lactoferrin increased the efficiency by 2-fold without enforced expression of Klf4. These results suggest that lactoferrin may induce dedifferentiation, at least partly by increasing the expression of Klf4.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.