Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide
- Authors
- Ma, Na; Hong, Seung In; Zhao, TingTing; No, Da Som; Kim, Chong-Tai; Kim, Yangha; Kim, In-Hwan
- Issue Date
- 3월-2014
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Docosahexaenoic acid (DHA); Ethanol Lipozyme RM IM (Rhizomucor miehei); Near-supercritical carbon dioxide; Tuna oil fatty acids
- Citation
- JOURNAL OF SUPERCRITICAL FLUIDS, v.87, pp.28 - 33
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF SUPERCRITICAL FLUIDS
- Volume
- 87
- Start Page
- 28
- End Page
- 33
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/99091
- DOI
- 10.1016/j.supflu.2013.12.024
- ISSN
- 0896-8446
- Abstract
- This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.