First Thermostable Endo-beta-1,4-Glucanase from Newly Isolated Xanthomonas sp EC102

Abstract

A novel gene encoding thermostable endoglucanase was identified in Xanthomonas sp. EC102 from soil. The gene had 1,458 base pairs of open reading frame, which encode a 52-kDa protein of 486 amino acid residues. Sequence of the amino acid residues was similar with the endoglucanase from Xanthomonas campestris pv. campestris ATCC33913 (GenBank Accession No. NP_638867.1) (94 % identity). The endoglucanase was overexpressed in Escherichia coli BL21 and purified. Temperature for the highest enzymatic activity was 70 A degrees C and pH optima was pH 5.5. The specific activity of the endoglucanase toward carboxymethylcellulose (CMC) was approximately 2 mu mol min(-1) mg(-1), V (max) for CMC was 1.44 mu mol mg(-1) min(-1), and K (m) values was 25.6 mg mL(-1). The EC102 endoglucanase was stable at temperatures up to 60 A degrees C, and it was activated by 0.1 mM of Mn2+ and Co2+. This is the first report about thermostable endoglucanase from Xanthomonas sp.