AMPK activator-mediated inhibition of endoplasmic reticulum stress ameliorates carrageenan-induced insulin resistance through the suppression of selenoprotein P in HepG2 hepatocytes
- Authors
- Jung, Tae Woo; Lee, So Young; Hong, Ho Cheol; Choi, Hae Yoon; Yoo, Hye Jin; Baik, Sei Hyun; Choi, Kyung Mook
- Issue Date
- 25-1월-2014
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Selenoprotein P; Insulin resistance; Hepatokine; AMPK; ER stress; Salsalate
- Citation
- MOLECULAR AND CELLULAR ENDOCRINOLOGY, v.382, no.1, pp.66 - 73
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR AND CELLULAR ENDOCRINOLOGY
- Volume
- 382
- Number
- 1
- Start Page
- 66
- End Page
- 73
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/99506
- DOI
- 10.1016/j.mce.2013.09.013
- ISSN
- 0303-7207
- Abstract
- Carrageenan (CGN) has been shown to cause inflammation through toll-like receptor 4, which may play an important role in insulin resistance and type 2 diabetes mellitus. Selenoprotein P (SeP) has recently been identified as a novel hepatokine that causes insulin resistance. Here, we report that treatment of HepG2 cells with CGN increased both CCAAT enhancer binding protein homologous protein (CHOP) and SeP expression. Pretreatment with 4-phenylbutyrate (4-PBA), an endoplasmic reticulum stress inhibitor, and PD98059, a c-Jun N-terminal kinase (JNK) inhibitor, reversed CGN-induced SeP expression. Moreover, both 4-PBA and knock-down of SeP improved CGN-induced insulin resistance. In addition, we found that adenosine monophosphate-activated protein kinase (AMPK) activators ameliorated CGN-induced insulin resistance in addition to suppressing CHOP and SeP expression. In conclusion, CGN-induced ER stress increased the expression of SeP through the JNK pathway, while AMPK activators ameliorated CGN-induced insulin resistance via SeP inhibition through the AMPK-mediated alleviation of ER stress in hepatocytes. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
- College of Medicine > Department of Medical Science > 1. Journal Articles
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