Demethylation of RUNX3 by Vincristine in Colorectal Adenocarcinoma Cells
- Authors
- Moon, Ji Wook; Lee, Soo Kyung; Lee, Jung Ok; Kim, Ji Hae; Kim, Nami; Kim, Jin; Kim, Hyeon Soo; Park, Sun-Hwa
- Issue Date
- 1월-2014
- Publisher
- INT INST ANTICANCER RESEARCH
- Keywords
- DNA methylation; vincristine; colonic neoplasms; demethylation; methylation-specific polymerase chain reaction; RUNX3
- Citation
- ANTICANCER RESEARCH, v.34, no.1A, pp.133 - 140
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANTICANCER RESEARCH
- Volume
- 34
- Number
- 1A
- Start Page
- 133
- End Page
- 140
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/99625
- ISSN
- 0250-7005
- Abstract
- Background: Methylation-mediated inactivation of tumor-suppressor genes is a critical event during the pathogenesis of many malignancies. Vincristine is a conventional anticancer drug used to treat various types of cancers. However, few studies describe the epigenetic-based effects of vincristine. In this study, changes in the methylation of runt-related transcription factor-3 (RUNX3) were investigated in CCD18Co normal colon cells and DLD-1 colorectal adenocarcinoma cells. Materials and Methods: CCD18Co and DLD-1 cells were treated with vincristine, and the methylation status was assessed using quantitative methylation-specific polymerase chain reaction (QMSP). Eleven normal colon tissues and 105 colorectal cancer tissues were investigated by methylation and mRNA expression of RUNX3 using QMSP and real-time reverse transcription polymerase chain reaction (real time-PCR). Results: RUNX3 was demethylated after vincristine treatment in DLD-1 cells. The expression of RUNX3 mRNA was down-regulated in DLD-1 cells because of DNA hypermethylation, but was restored after vincristine treatment. In addition, hypermethylation of RUNX3 was detected in 70 out of 105 colorectal carcinomas (66.7%). RUNX3 hypermethylation was greater in colon cancer tissues than in rectal cancer tissues. The expression of RUNX3 mRNA was reduced in 68 out of 105 colorectal cancer tissues (64.8%). Conclusion: These results demonstrate that vincristine demethylates RUNX3 in colorectal adenocarcinoma cells, and restores its expression.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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