Peyer's patch-mediated intestinal immune system modulating activity of pectic-type polysaccharide from peel of Citrus unshiu
- Authors
- Suh, Hyung-Joo; Yang, Hyun-Seuk; Ra, Kyung-Soo; Noh, Dong-Ouk; Kwon, Ki-Han; Hwang, Jong-Hyun; Yu, Kwang-Won
- Issue Date
- 1-6월-2013
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Citrus unshiu peel, Pectic-type polysaccharide; Base-catalysed beta-elimination; Peyer' s patch; Intestinal immune system modulating activity
- Citation
- FOOD CHEMISTRY, v.138, no.2-3, pp.1079 - 1086
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOOD CHEMISTRY
- Volume
- 138
- Number
- 2-3
- Start Page
- 1079
- End Page
- 1086
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/102992
- DOI
- 10.1016/j.foodchem.2012.11.091
- ISSN
- 0308-8146
- Abstract
- An intestinal immune system modulating polysaccharide (CUI-3IIb-3-2, 18 kDa) was purified from Citrus unshiu peel. CUI-3IIb-3-2 mainly comprised GalA, GlcA, Ara, Gal and Rha, and it consisted of 4-linked GalA, terminal Araf, 4- or 5-linked/3,4- or 3,5-branched Ara, terminal Gal, and 2-linked/2,4-branched Rha. After CUI-3IIb-3-2 digestion by endo-alpha-D-(1 -> 4)-polygalacturonase, its hydrolysate was fractionated into PG-1 and PG-2. Methylation analyses of PG-1 and PG-2 using base-catalysed beta-elimination suggested that CUI-3IIb-3-2 be assumed as pectic-type polysaccharide. Since the activities of PG-1 and PG-2 were potently decreased, the whole polysaccharide structure of CUI-3IIb-3-2 would be essential to maintain the activity. Meanwhile, when CUI-3IIb was orally administered in mice, bone marrow cell proliferation and GM-CSF/IL-6 production from Peyer's patch cell were significantly higher (1.76- and 2.03/2.51-fold, respectively) than a saline. Therefore, a pectic-type polysaccharide from citrus peel could stimulate Peyer's patches and produce hematopoietic growth factors resulted in bone marrow cell proliferation. (C) 2012 Elsevier Ltd. All rights reserved.
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