Enzyme-conjugated ZnO nanocrystals for collisional quenching-based glucose sensing
- Authors
- Kim, Ki-Eun; Kim, Tae Geun; Sung, Yun-Mo
- Issue Date
- 2012
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- CRYSTENGCOMM, v.14, no.8, pp.2859 - 2865
- Indexed
- SCIE
SCOPUS
- Journal Title
- CRYSTENGCOMM
- Volume
- 14
- Number
- 8
- Start Page
- 2859
- End Page
- 2865
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/109365
- DOI
- 10.1039/c2ce06410c
- ISSN
- 1466-8033
- Abstract
- A simple approach to sensitive glucose detection has been developed based upon variation in the fluorescence of ZnO nanocrystals with glucose concentration. ZnO nanocrystals were successfully synthesized in wurtzite structure using a surfactant, mercaptoundecanoic acid (MUA) via the polyol method. MUA molecules not only served as a template for the synthesis of spherical-shape nanoparticles but also provided water solubility and biocompatibility due to its carboxyl group. Carboxyl-terminated ZnO nanocrystals were activated by esterification of n-hydroxysulfosuccinimide (Sulfo-NHS) catalyzed by water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Glucose oxidase (GOx), an enzyme could be immobilized to ZnO nanocrystals by replacing NHS with amino-acid groups of GOx. ZnO-MUA-GOx bioconjugates showed a decrease in the photoluminescence (PL) intensity by appearance of glucose molecules due to the collisional quenching by hydrogen peroxide generated from enzymatic oxidation reaction of glucose. PL intensity showed a linear decrease with glucose concentration from 1.6 to 33.3 mM, which fully covers the physiological glucose level. ZnO-MUA-GOx bioconjugates showed a detection limit lower than 0.33 mM and a response time less than 5 s. They also revealed distinct specificity against cholesterol molecules.
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Collections - College of Engineering > School of Electrical Engineering > 1. Journal Articles
- College of Engineering > Department of Materials Science and Engineering > 1. Journal Articles
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